TOP1 (1291CT875.142.166) Monoclonal Antibody
#
-
Catalog numberbsm-51396M
-
Price:
-
Size0.1ml
-
-
Long nameTOP1 (1291CT875.142.166) Monoclonal Antibody
-
Also known asTOP1 (1291CT875.142.166) Monoclonal Antibody
-
CategoryPrimary Antibodies
-
ConjugationUnconjugated
-
Target AntigenTOP1
-
SpecificityThis is a highly specific antibody against TOP1.
-
Modification sNone
-
Modification site sUnmodified antibody
-
ClonalityMonoclonal
-
Clone number1291CT875.142.166
-
SourceThis TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
-
Gene ID number7150
-
Swiss ProtP11387
-
Tested ApplicationsWB, FCM
-
Recommended dilutionsWB(1:1000), FC(1:25),
-
Cross reactivityHuman, Rat
-
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the target antigenReleases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
-
Purification methodPurified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
-
Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
-
Synonym namesTOPI; DNA topoisomerase 1; DNA topoisomerase I; TOP1
-
PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translationanticorps
-
Gene target
-
Gene symbolTOP1, PLCG1-AS1
-
Short nameAnti-TOP1
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
HostMouse (Mus musculus)
-
IsotypeImmunoglobulin G (IgG)1 κ
-
Alternative nameTOP1 (1291CT875.142.166) Monoclonal Antibody
-
Alternative techniqueantibodies
-
Alternative to gene targettopoisomerase (DNA) I, TOPI, TOP1 and IDBG-75139 and ENSG00000198900 and 7150, poly(A) RNA binding, nuclei, Top1 and IDBG-211615 and ENSMUSG00000070544 and 21969, BT.39255 and IDBG-642660 and ENSBTAG00000007960 and 534799
-
Gene info
-
Identity
-
Gene
-
Long gene nameDNA topoisomerase I
-
Synonyms gene name
- topoisomerase (DNA) I
-
Synonyms name
-
Locus
-
Discovery year1989-02-23
-
Entrez gene record
-
Classification
- Topoisomerases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namePLCG1 antisense RNA 1
-
Synonyms
-
Locus
-
Discovery year2014-06-13
-
Entrez gene record
-
Classification
- Antisense RNAs
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data