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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Goat anti-Mouse IgG (H&L) - Affinity Pure]
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Other_names
[IgG, partial]
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Reactivity
Mouse. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum immunoglobulins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Mouse IgG.
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Form
Clear, colorless liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify Goat anti-Mouse IgG (H L) - Pure, Conjugate by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Mouse IgG (H L) - Pure, Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Mus musculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
Goat anti-Mouse IgG (H L) - Pure, Conjugate
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Technique
affinity, anti-, Mouse, anti, Goat, Pure, IgG, antibody to, antibodies, goats, IgGs, mouses, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Biotin
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Species
Mouse, Goats, Mouses
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Alternative name
caprine Antibody toMouse Immunoglobulin G (heavy + light chain) - protein Pure, biotinilated Conjugate
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Alternative technique
murine, antibodies, purified, caprine, immunoglobulins
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