Mouse anti Cytokeratin 10 / Keratin K10

• Catalog number
  MUB0319P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  RKSE60 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with Cytokeratins from the Human epidermis.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  RKSE60
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 10 / Keratin K10 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Canine,Human,Mouse,Rat,Swine,Zebrafish
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  RKSE60 reacts exclusively with Cytokeratin 10 which is present in Keratinizing stRatified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
• Research interest
  Cytoskeleton
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
• Antibody s suited for
  RKSE60 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Ramaekers, F. C., Puts, J. J., Moesker, O., Kant, A., Huysmans, A., Haag, D., Jap, P. H., Herman, C. J., and Vooijs, G. P. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview, Histochem J 15, 691-713. Puts, J. J., Moesker, O., Kenemans, P., Vooijs, G. P., and Ramaekers, F. C. (1985). Expression of Cytokeratins in early neoplastic epithelial lesions of the uterine cervix, Int J Gynecol Pathol 4, 300-13. Broers, J. L., Carney, D. N., Klein Rot, M., Schaart, G., Lane, E. B., Vooijs, G. P., and Ramaekers, F. C. (1986). Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies, J Cell Sci 83, 37-60. Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of Keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45. Bijman, J.T., Wagener, D.J.T., van Rennes, H., Wessels, J.M.C., Ramaekers, F.C.S. and van den Broek, P. (1987). Modulation of placental alkaline phosphatase activity and Cytokeratins in Human HN-1 cells by butyRate, retinoic acid, Catecholamines and histamine, Br. J. Cancer 56, 127-32. Van Erp, P.E.J., Rijzewijk, J.J., Boezeman, J.B.M., Leenders, J., de Mare, S., Schalkwijk, J., van de Kerkhof, P.C.M., Ramaekers, F.C.S. and Bauer, F.W. (1989). Flow Cytometric analysis of epidermal subpopulations from normal and psoriatic skin using monoclonal antibodies anti intermediate filaments, Am J Pathol 135, 865-70. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of Cytokeratin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. Franssen, M.E.J., Boezeman, J.B.M., van de Kerkhof, P.C.M. and van Erp, P.E.J. (2003). Monitoring hyperprolifeRative disorders in Human skin: flow cytometry of changing Cytokeratin expression, Cytometry Part B (Clinical Cytometry) 57B, 32-39.
• Protein number
  P13645
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin   K10  
• Gene symbol
  KRT10
• Short name
  Mouse anti Cytokeratin 10 / Keratin K10
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 10 / Keratin K10
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6413
• Gene
  KRT10
• Long gene name
  keratin 10
• Synonyms gene
  • KPP
• Synonyms gene name
  • keratosis palmaris et plantaris
  • keratin 10, type I
• Synonyms
  • K10
  • CK10
• Synonyms name
  • cytokeratin 10
  • epidermolytic hyperkeratosis
• GenBank acession
  • J04029
• Locus
  17q21.2
• Discovery year
  1988-08-12
• Entrez gene record
  3858
• Pubmed identfication
  • 2461420
  • 16831889
• RefSeq identity
  • NM_000421
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133368

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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