BTF Antibody

• Catalog number
  3743-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  BTF
• Target Alternative Name
  BTF, BCLAF1, BCLAF-1, BCL2-Associated Transcription Factor 1, KIAA0164
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Synthetic peptide of human BTF
• Accession
  NP_055554
• Gene Id
  9774
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation
• Application And Usages
  Western blotting (1:500-1:2000) and immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control for Western blotting. The antibody recognizes the BTF of human and mouse origins. Reactivity to other species has not been tested.
• Description
  The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II, the transcription factors class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box. An additional TFII related protein, BTF3, does not directly associate with the proximal promoter, but rather forms a stable complex with RNA pol II and facilitates RNA pol II assembling into the complex. The BTF3 gene is ubiquitously expressed and encodes for two protein isoforms, BTF3a and BTF3b, which are produced from alternative splicing. The BTF3 proteins are identical except that BTF3b lacks the first 44 amino acids at the N-terminal of BTF3a. As a consequence of this deletion, BTF3b is unable to induce transcription, despite being able to bind RNA pol II. Additionally, BTF3a and BTF3b associate with the widely expressed protein kinase CK2. CK2 phosphorylates BTF3a, as well as TFIIB, and is required for the efficient transcription of the tRNA and 55 rRNA genes by RNA pol III. BTF3 belongs to the NAC-β family, which includes several related proteins, such as BTF3L1, BTF3L2, BTF3L3 and BTF3L4.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  BTF  
• Gene symbol
  BCLAF1
• Short name
  BTF Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  BTF (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16863
• Gene
  BCLAF1
• Long gene name
  BCL2 associated transcription factor 1
• Synonyms gene name
  • BCL2-associated transcription factor 1
• Synonyms
  • KIAA0164
  • BTF
• Synonyms name
  • BCLAF1 and THRAP3 family member 1
• GenBank acession
  • AF249273
• Locus
  6q23.3
• Discovery year
  2004-01-13
• Entrez gene record
  9774
• Pubmed identfication
  • 8724849
  • 10330179
• RefSeq identity
  • NM_014739
• Classification
  • WTAP complex
• VEGA ID
  OTTHUMG00000033323

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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