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Product Type
Cell Lysate
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Concentration
2mg/ml (determined by BCA assay)
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Formulation
Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
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Applications
WB
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WB Comment and Pic
All lanes : HepG2 Whole Cell Lysate (40ug);;Lane 1. : Anti-GAPDH antibody (ATGA0181);;Lane 2. : Anti-Beta tubulin antibody (ATGA0196) {ATGL0005-WB.jpg}
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Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
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Antigen Species
Human
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Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
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Storage and shipping
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
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