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Frizzled 5/8 Antibody

Frizzled 5/8 Antibody is available 1 time from Bioss primary unconjugated antibodies labs

bs-2930R | Frizzled 5/8 Antibody size: 0.1ml | 308.79 USD

Catalog number bs-2930R
Price 308.79 USD
Size 0.1ml
Product images
    #.pngL1 rat lung lysates, L2 rat brain lysates probed (bs-2930R) Anti-Frizzled-5 Polyclonal, Unconjugated at 1:200 in 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37?C. Predicted band and observed band size: 62kD.Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.K562 cell lysates probed with Rabbit Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:300 overnight at 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37?C.
Product files
Long name Frizzled 5/8 Primary Polyclonal Antibody
Also known as Frizzled 5/8 PAb
Category Primary Antibodies
Conjugation Unconjugated
Target Antigen Frizzled 5/8
Specificity This is a highly specific antibody against Frizzled 5/8.
Modification(s) None
Modification site(s) Unmodified antibody
Clonality Polyclonal antibody
Clone number Polyclonal antibody
Concentration 1ug per 1ul
Immunogen range 27-77/585
Subcellular location Extracellular
Source This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Frizzled 5
Gene ID number 7855, 8325
Tested Applications WB, IHC-P, IF(IHC-P)
Recommended dilutions WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Cross reactivity Human, Mouse, Rat
Cross reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigen Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 5 protein is believed to be the receptor for the Wnt5A ligand. Frizzled 5 has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. Frizzled 5 was cloned from a retina cDNA library. Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
Purification method This antibody was purified via Protein A.
Storage conditions Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Synonym names C2orf31; Frizzled homolog 5; Frizzled-5; Frizzled5; Fz 5; Fz-5; Fz5; FZD 5; hFz5; FZD5; FZD5_HUMAN; FzE 5; FzE5; HFZ 5; Seven transmembrane receptor frizzled 5; Wnt receptor; hFz8; Fz-8; Frizzled-8; FZD8.
Properties If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps
Gene targetFrizzled 5/8
Short name Anti-Frizzled 5/8
Technique Antibody
Host Rabbit (Oryctolagus cuniculus)
Isotype Immunoglobulin G (IgG)
Alternative name Frizzled 5/8 Polyclonal Antibody
Alternative technique antibodies
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Anti-Frizzled 5/8 | Technique alternative | 01010351884
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