PIM2, Active
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Catalog number7730-5
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PricePlease ask
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Size5 ug
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SynonymsPIM, Serine/threonine-protein kinase Pim-2
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Alternative_namesPIM, Serine/threonine-protein kinase Pim-2
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DescriptionA proto-oncogene
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RecombinantYes
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SourceBaculovirus (Sf9 insect cells)
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test method305 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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Molecular Weight61.0 kDa
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Storage Temp-80°C
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Shippingdry ice
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Shelf Life12 months
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Concentration5µg/50 µl
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AppearanceLiquid
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Physical form descriptionRecombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background InformationBaytel et al. identified a deduced 334-amino acid sequence of the clone, which showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. The results suggested a role for PIM2 in proliferating cells as well as during meiosis (1). Yan et al investigated potential functions for the pim family of kinases in factor-dependent murine hematopoietic cells and indicate that pim-2 functions similarly to pim-1 as a pro-survival kinase and suggest that BAD is a legitimate PIM-2 substrate (2). Hammerman et al concluded that the transcriptional induction of Pim-2 initiated a novel NF-kappaB activation pathway that regulates cell survival (3).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolPIM2
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Short namePIM2, Active
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Alternative namepim-2 oncogene, functionnal
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Alternative to gene targetpim-2 oncogene, PIM2 and IDBG-64771 and ENSG00000102096 and 11040, transferase activity, Plasma membranes, Pim2 and IDBG-129634 and ENSMUSG00000031155 and 18715, PIM2 and IDBG-636953 and ENSBTAG00000004597 and 508424
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Gene info
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Identity
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Gene
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Long gene namePim-2 proto-oncogene, serine/threonine kinase
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Synonyms gene name
- pim-2 oncogene
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GenBank acession
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Locus
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Discovery year1999-08-26
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases