CD3, epsilon (Early T Cell Marker) (FITC)

• Catalog number
  GEN632841
• Price
  Please ask
• Size
  100ug

• Also known as
  CD3, epsilon
• Other names
  CD3 epsilon; N/A; CD3 epsilon; N/A; N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  cd3e; N/A; N/A; N/A
• Gene name synonims
  cd3e; N/A; N/A; N/A
• Other gene names
  cd3e; N/A; N/A; N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG,k
• Clone
  145-2C11
• Host organism
  Hamster
• Source organism
  Mouse (Mus musculus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Purified
• Form Appearance
  Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Conjugated to FITC.
• Concentration
  N/A
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested applications
  Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  CD3   epsilon   Early  
• Short name
  CD3, epsilon (Early T ) (FITC)
• Technique
  FITC, Fluorescein, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
• Label
  FITC
• Alternative name
  Anti-CD3, epsilon (Early T Cell Marker) (FITC)
• Alternative technique
  fluorescine
• Tissue
  cell

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products