Rat Anti-Mouse CD24/HSA-FITC

• Catalog number
  GEN673592
• Price
  Please ask
• Size
  100ug

• Also known as
  CD24/HSA
• Other names
  N/A
• Category
  Secondary Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Rat IgG2c kappa
• Clone
  30-F1
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Mouse CD24/heat stable antigen (HSA) CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70 kDa.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti- CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Fluorescein (FITC) Conjugate
• Concentration
  N/A
• Storage and shipping
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested applications
  Flow Cytometry; Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD24/HSA  
• Gene symbol
  CD24, MIR3591, MIR3150A, MIR146A, MIR374A, MIR18A, MIR301A, MIR148A, MIR323B, MIR193A
• Short name
  Anti-Mouse CD24/HSA-FITC
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  Rat
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Anti-Rat Anti-Mouse CD24/HSA-FITC
• Alternative technique
  murine, antibodies, fluorescine

Gene info

• Identity
  HGNC:1645
• Gene
  CD24
• Long gene name
  CD24 molecule
• Synonyms gene name
  • CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• Synonyms
  • CD24A
• Locus
  6q21
• Discovery year
  1994-09-07
• Entrez gene record
  100133941
• Pubmed identfication
  • 7959762
• RefSeq identity
  • NM_013230
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000188439

Gene info

• Identity
  HGNC:41875
• Gene
  MIR3591
• Long gene name
  microRNA 3591
• Synonyms
  • hsa-mir-3591
  • hsa-mir-122b
• Locus
  18q21.31
• Discovery year
  2011-05-02
• Entrez gene record
  100616357
• RefSeq identity
  • NR_039899
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:38362
• Gene
  MIR3150A
• Long gene name
  microRNA 3150a
• Synonyms gene
  • MIR3150
• Synonyms gene name
  • microRNA 3150
• Synonyms
  • hsa-mir-3150
  • hsa-mir-3150a
• Locus
  8q22.1
• Discovery year
  2010-04-30
• Entrez gene record
  100422964
• RefSeq identity
  • NR_036105
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:31533
• Gene
  MIR146A
• Long gene name
  microRNA 146a
• Synonyms gene
  • MIRN146
  • MIRN146A
• Synonyms gene name
  • microRNA 146
• Synonyms
  • hsa-mir-146
  • hsa-mir-146a
• Locus
  5q33.3
• Discovery year
  2004-04-23
• Entrez gene record
  406938
• RefSeq identity
  • NR_029701
• Classification
  • MicroRNA MIR146 family

Gene info

• Identity
  HGNC:31788
• Gene
  MIR374A
• Long gene name
  microRNA 374a
• Synonyms gene
  • MIRN374
  • MIRN374A
• Synonyms gene name
  • microRNA 374
• Synonyms
  • hsa-mir-374
  • hsa-mir-374a
• Locus
  Xq13.2
• Discovery year
  2004-07-22
• Entrez gene record
  442919
• RefSeq identity
  • NR_030785
• Classification
  • MicroRNA MIR374 family

Gene info

• Identity
  HGNC:31548
• Gene
  MIR18A
• Long gene name
  microRNA 18a
• Synonyms gene
  • MIRN18
  • MIRN18A
• Synonyms gene name
  • microRNA 18
• Synonyms
  • hsa-mir-18
  • hsa-mir-18a
• Locus
  13q31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406953
• RefSeq identity
  • NR_029488
• Classification
  • MicroRNA MIR17 family

Gene info

• Identity
  HGNC:31622
• Gene
  MIR301A
• Long gene name
  microRNA 301a
• Synonyms gene
  • MIRN301
  • MIRN301A
• Synonyms gene name
  • microRNA 301
• Synonyms
  • hsa-mir-301
  • hsa-mir-301a
• Locus
  17q22
• Discovery year
  2004-04-23
• Entrez gene record
  407027
• RefSeq identity
  • NR_029842
• Classification
  • MicroRNA MIR130 family

Gene info

• Identity
  HGNC:31535
• Gene
  MIR148A
• Long gene name
  microRNA 148a
• Synonyms gene
  • MIRN148
  • MIRN148A
• Synonyms gene name
  • microRNA 148
• Synonyms
  • hsa-mir-148
  • hsa-mir-148a
• Locus
  7p15.2
• Discovery year
  2004-04-23
• Entrez gene record
  406940
• RefSeq identity
  • NR_029597
• Classification
  • MicroRNA MIR148/152 family

Gene info

• Identity
  HGNC:38349
• Gene
  MIR323B
• Long gene name
  microRNA 323b
• Synonyms gene
  • MIR453
  • MIRN453
• Synonyms gene name
  • microRNA 453
• Synonyms
  • hsa-mir-323b
  • hsa-mir-453
• Locus
  14q32.31
• Discovery year
  2010-04-30
• Entrez gene record
  574410
• RefSeq identity
  • NR_029969
• Classification
  • MicroRNA MIR154 family

Gene info

• Identity
  HGNC:31563
• Gene
  MIR193A
• Long gene name
  microRNA 193a
• Synonyms gene
  • MIRN193
  • MIRN193A
• Synonyms gene name
  • microRNA 193
• Synonyms
  • hsa-mir-193
  • hsa-mir-193a
• Locus
  17q11.2
• Discovery year
  2004-04-23
• Entrez gene record
  406968
• RefSeq identity
  • NR_029710
• Classification
  • MicroRNA MIR193 family

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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