Biotin Conjugated Mouse Anti-human IgG antibody
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Catalog number
BM2001-1
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Price
Please ask
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Size
1000 µl
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Type of the antibody protein
IgG secondary antibody
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Raised in synthesized
Mouse
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Conjugation
Biotin
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Product configuration
Solution with concentration 1mg/ml
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Immunogen
Human IgG (whole molecule)
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Clonality
Polyclonal antibodies
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Analyses
IHC-P,IHC-F,ICC,WB,ELISA
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Storage conditions
Keep the Biotin Conjugated Mouse Anti-human IgG antibody at plus four degrees Celsius for 12 months.
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Product details
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all mouse serum proteins, except the specific antibody for human IgG.
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Specificity
This biotin conjugated antibody is specific for human IgG and shows no cross-reactivity with rabbit/goat/bovine IgG.
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Tips
The Biotin Conjugated Mouse Anti-human IgG antibody is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
boster supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Additional conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Short name
Conjugated Mouse Anti- IgG antibody
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Technique
anti-Human, Antibody, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, antibody Conjugates, IgGs, mouses
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Host
mouse
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Isotype
IgG, IgG
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Label
Biotin
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Species
Human, Humans, Mouses
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Alternative name
biotinilated coupled Mouse Antibody tohuman Immunoglobulin G (antibody to-)
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Alternative technique
antibodies, murine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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