Mouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b, Clone 6F10
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Catalog numberSMC-510D-BI
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PricePlease ask
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Size100 µg
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Clone6F10
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ImmunogenSynthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
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Antibody s full descriptionMouse Anti- 4-Hydroxy-2-hexenal Monoclonal IgG2b Antibody, Clone: 6F10: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other name4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody
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Raised inMouse
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Antibody s target4-Hydroxy-2-hexenal
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Primary research fieldsCancer, Oxidative Stress, Lipid peroxidation
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BrandnameMOLECULAR SIGNATURE®
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Antibodies applicationsWB, ICC/IF, ELISA
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Antibody s reactivitySpecies Independent
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Antibody s dilutionsWB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration1 mg/ml
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Antibody s specificitySpecific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:1000 dilution of SMC-510 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cellSee included datasheet or contact our support service
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Tissue specificitySee included datasheet or contact our support service
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Released date23-Mar-2017
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10 (SMC-510). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody (SMC-510) at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) 4-Hydroxy-2-hexenal Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal-BSA using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10 (SMC-510). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody (SMC-510) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-4-Hydroxy-2-hexenal Antibody [6F10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-510) Mouse Anti-4-Hydroxy-2-hexenal Antibody [6F10] used in Western Blot (WB) on 4-hydroxy-2-hexanal-BSA Conjugate (SMC-510)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolTRL-AAG2-4, MIR3130-2, TRP-AGG2-4, TRG-TCC2-4, TRD-GTC2-4, TRG-GCC2-4, TRN-GTT2-4, TRR-ACG2-4, CHST9, TRM-CAT4-2
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Short nameMouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b, Clone 6F10
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelBiotin
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody to4-Hydroxy-2-hexenal (4-HHE) monoclonal IgG2b, clonality 6F10
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Alternative techniquemurine, antibodies
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Clone name6F10
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Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon AAG) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 27 (anticodon AAG)
- transfer RNA-Leu (AAG) 2-2
- transfer RNA-Leu (AAG) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namemicroRNA 3130-2
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Synonyms gene
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Synonyms gene name
- microRNA 3130-4
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Synonyms
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Locus
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Discovery year2010-04-30
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Pro (anticodon AGG) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA proline 6 (anticodon AGG)
- transfer RNA-Pro (AGG) 2-2
- transfer RNA-Pro (AGG) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon TCC) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 17 (anticodon UCC)
- transfer RNA-Gly (TCC) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Asp (anticodon GTC) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA aspartic acid 6 (anticodon GUC)
- transfer RNA-Asp (GTC) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon GCC) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 6 (anticodon GCC)
- transfer RNA-Gly (GCC) 2-2
- transfer RNA-Gly (GCC) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-20
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Asn (anticodon GTT) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA asparagine 7 (anticodon GUU)
- transfer RNA-Asn (GTT) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Arg (anticodon ACG) 2-4
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Synonyms gene
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Synonyms gene name
- transfer RNA arginine 29 (anticodon ACG)
- transfer RNA-Arg (ACG) 2-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namecarbohydrate sulfotransferase 9
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Synonyms gene name
- carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-11-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sulfotransferases, membrane bound
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Met (anticodon CAT) 4-2
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Synonyms gene
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Synonyms gene name
- transfer RNA methionine 19 (anticodon CAU)
- transfer RNA-Met (CAT) 4-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data