ASH1, Histone-lysine N-methyltransferase ASH1
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Catalog numberAS14 2816
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PricePlease ask
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Size50 µg
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Available ordering formatLyophilized in PBS pH 7.4
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ImmunogenN-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteFor reconstitution add 50 µl of sterile water.
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB), immunolocalization (IL), chromatin immunoprecipitation (ChIP)
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Connected productsSecondary antibodies
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Recommended dilutions for use1: 1000 (WB), 1:500 (IL), 10ng/ul (ChIP)
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Molecular weight expected аpparent236 | 300 kDa
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Verified reactivityDrosophila melanogaster
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Possible reactivityDrosophila melanogaster
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No reactivityno confirmed exceptions from predicted reactivity are currently known
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Supplementary informationto be added when available
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ReferencesKahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print]. Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
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Scientific contextHistone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
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NotesNone
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Protein numberQ9VW15
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TAIR numberRefer to NCBI
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Gene target
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Gene symbolASCL1, ASH1L
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Short nameASH1, Histone-lysine N-methyltransferase ASH1
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Alternative nameASH1, Histone-lysine N-methyltransferase ASH1
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Gene info
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Identity
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Gene
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Long gene nameachaete-scute family bHLH transcription factor 1
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Synonyms gene name
- achaete-scute complex (Drosophila) homolog-like 1
- achaete-scute complex-like 1 (Drosophila)
- achaete-scute complex homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-09
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Entrez gene record
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Pubmed identfication
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameASH1 like histone lysine methyltransferase
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Synonyms gene name
- ash1 (absent, small, or homeotic)-like (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Bromodomain containing
- PHD finger proteins
- SET domain containing
- Lysine methyltransferases
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VEGA ID