Recombinant Human Protein N-terminal Glutamine Amidohydrolase/NTAQ1 Protein 50µg
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Catalog number
PKSH032966-50µg
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Price
Please ask
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Size
50µg
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Synonym
Protein N-terminal glutamine amidohydrolase;WDYHV1;Protein NH2-terminal glutamine deamidase;N-terminal Gln amidase;Nt(Q)-amidase;C8orf32; NTAQ1
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Activity
NA
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Sequence
Met1-Cys205
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Fusion tag
N-GST
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Accession
Q96HA8
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Expressed Host
E.coli
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
>95% as determined by reducing SDS-PAGE.
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Endotoxin
<1.0 EU per µg as determined by LAL test.
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Stability and Storage
Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
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Mol Mass
49.8 kDa
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AP Mol Mass
45-50 kDa
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Formulation
Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
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Reconstitution
Please refer to it for detailed information.
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Background
Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NTAQ1
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Short name
Recombinant Protein N-terminal Glutamine Amidohydrolase/NTAQ1 Protein 50µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Protein N-terminal Glutamine Amidohydrolase/NTAQ1 Protein 50µg
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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