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Category
Secondary Antibodies
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Long description
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. This IgG preparation is intended for use in precipitating (gel diffusion) and nonprecipitating antibody-binding immunoassays (indirect immunofluorescence, enzyme immunoassays for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids.; to prepare an insoluble immunoaffinity adsorbent or a solid phase catching antibody reagent by coupling to an artificial carrier; for labeling with a marker of the user’s own choice. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
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Antibody come from
A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity
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Clone
Polyclonal
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Antigen antibody binding interaction
Goat anti Human Ig kappa light chain (free and bound) Antibody
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Antibody is raised in
Goat
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Antibody s reacts with
Human
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Antibody s reacts with these species
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
IgG fraction of polyclonal Goat antiSerum to free and bound Human Ig kappa light chain
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Research interest
Immunology
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Application
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
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Antibody s suited for
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Precipitin titre not less than 1:64 when tested against normal human serum in agar immunodiffusion block titration.
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Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin name
Capra aegagrus hircus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
