Active Cathepsin D, Human Recombinant

• Catalog number
  9229-50
• Price
  Please ask
• Size
  50 µg

• Synonyms
  Procathepsin D, CTSD
• Alternative_names
  Procathepsin D, CTSD
• Description
  An active Lysosomal aspartyl protease
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥80%
• Assay
  SDS-PAGE
• Purity
  ≥80%
• Activity Specifications test method
  Measured by Cathepsin D Activity Fluorometric Assay Kit and Pepstatin A inhibition using Cathepsin D inhibitor screening kit
• Biological activity
  specific activity >100 pmol/min/mg and IC50 = 0.32 pM
• Results
  BV's Cathepsin D has specific activity >100 pmol/min/mg and IC50 = 0.32 pM
• Molecular Weight
  45.1 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  6 months
• Appearance
  Freeze-Dried
• Physical form description
  Freeze-dried from proprietary buffer
• Reconstitution Instructions
  Reconstitute to 1 mg/ml in water
• Background Information
  Cathepsin D is a lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node negative and node positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer’s plaques. Cathepsin D is produced initially as a pre-pro-enzyme which gets transported to lysosomes via endosomes in most cell types. In endosomes, it gets proteolyzed by unidentified proteases by removal of the pro-peptide to generate active single-chain Cathepsin D; while in lysosomes, further processing by cysteine cathepsins B and L generates mature, active double-chain Cathepsin D. BioVision’s Active Human Cathepsin D is a proteolytically active, non-glycosylated enzyme expressed, purified and activated by proprietary methods.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   Cathepsin  
• Short name
  Active Cathepsin D, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  functionnal Cathepsin D, H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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