Active Cathepsin B, human recombinant
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Catalog number
7580-50
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Price
Please ask
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Size
50 ug
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Synonyms
CTSB, CPSB
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Alternative_names
CTSB, CPSB
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Description
A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
>7500 mU/mg (1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100)).
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Biological activity
>7500 mU/mg (1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100)).
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Results
>7500 mU/mg
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Unit Definition
1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100).
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Molecular Weight
27.8 kDa (80-333 aa)
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Concentration
0.5 mg/ml
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Appearance
Liquid
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Physical form description
0.5 mg/ml solution of protein in 25 mM sodium acetate, 50 mM NaCl, pH 5.0 containing 50% glycerol.
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Background Information
Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Active Cathepsin B, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
functionnal Cathepsin B, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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