Rat HSP-27/HSPB1(Heat Shock Protein 27) EIA Kit
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Catalog numberE-EL-R0476-96T
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PricePlease ask
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Size96Test
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Detection TypeColormetric
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Target SpeciesRat
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Target SynonymHSPB1, CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-27/HSPB1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-27/HSPB1. You can calculate the concentration of Rat HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity0.47ng/mL
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Detection Range0.78~50ng/mL
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Target NameHSP-27/HSPB1
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UNIProt IDP42930
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameRat HSP-27/HSPB1(Heat Shock Protein 27) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolHSPB1, SNORD115-27, IGKV1D-27, ERVK-27, ERVW-27, RNVU1-27, IGHV7-27, SNORD116-27, SNORD114-27, IGLV3-27
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Short nameHSP-27/HSPB1(Heat Shock Protein 27) EIA Kit
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TechniqueEIA, eia
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HostRat
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SpeciesRat, Rats
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Alternative nameRat HSP-27/heat shock 27kDa protein 1(Heat Shock Protein 27) EIA reagent
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Alternative techniquekits, elisas
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 1
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Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-09
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-27
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-27 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-27
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 27
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Synonyms gene name
- endogenous retrovirus group K, member 27
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 27
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Synonyms gene name
- endogenous retrovirus group W, member 27
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 27
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 7-27 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 7-27
- immunoglobulin heavy variable 7-27 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-27
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-27
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-27
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data