Rat Anti-Mouse CD3e-CY5

• Catalog number
  GEN670329
• Price
  Please ask
• Size
  100ug

• Also known as
  CD3e-CY5
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2b, kappa
• Clone
  C363.29B (YCD3-1)
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Mouse CD3epsilon (Mr 25 kD) CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.1, 2 CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.1, 3, 4 The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.1; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Cyanine 5 (CY 5) Conjugate
• Concentration
  N/A
• Storage and shipping
  The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..
• Tested applications
  Identification and enumeration of CD3+ cells by Flow Cytometry 1, 2, Immunoprecipitation 1, In vitro depletion of CD3+ cells 1, In vitro activation of T cells (accessory cells must be provided) 1, 3, 4 Immunohistochemistry (PS)5
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
• Conjugation
  Cy5.5
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD3e  
• Gene symbol
  CD3E, POLR1G
• Short name
  Anti-Mouse CD3e-
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Rat
• Label
  Cy5
• Species
  Mouse, Mouses, Rats
• Alternative name
  Anti-Rat Anti-Mouse CD3e-CY5
• Alternative technique
  murine, antibodies
• Alternative to gene target
  CD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054

Gene info

• Identity
  HGNC:1674
• Gene
  CD3E
• Long gene name
  CD3e molecule
• Synonyms gene name
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
• GenBank acession
  • X03884
• Locus
  11q23.3
• Discovery year
  1986-01-01
• Entrez gene record
  916
• RefSeq identity
  • NM_000733
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000166968
• Locus Specific Databases
  • CD3Ebase: Mutation registry for autosomal recessive CD3epsilon immunodeficiency
  • LRG_38

Gene info

• Identity
  HGNC:24219
• Gene
  POLR1G
• Long gene name
  RNA polymerase I subunit G
• Synonyms gene
  • CD3EAP
• Synonyms gene name
  • CD3e antigen, epsilon polypeptide associated protein
  • CD3e molecule associated protein
• Synonyms
  • ASE-1
  • CAST
  • PAF49
  • RPA34
  • A34.5
• Synonyms name
  • CD3 epsilon associated protein
  • antisense to ERCC 1
• GenBank acession
  • U86751
• Locus
  19q13.32
• Discovery year
  2005-04-21
• Entrez gene record
  10849
• Pubmed identfication
  • 10373416
  • 9426281
  • 15226435
  • 23150253
• RefSeq identity
  • NM_012099
• Classification
  • RNA polymerase subunits
• VEGA ID
  OTTHUMG00000182120

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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