Mouse Anti-Mouse FIG4/SAC3 Monoclonal IgG1, Clone S202-7
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Catalog numberSMC-468D-A680
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PricePlease ask
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Size100 µg
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CloneS202-7
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ImmunogenFusion protein amino acids 688-907 (C-terminus) of mouse Sac3. Rat: 95% identity (203/220 amino acids identical). Human: 91% identity (201/220 amino acids identical)
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Antibody s full descriptionMouse Anti-Mouse FIG4 phosphatase Monoclonal IgG1 Antibody, Clone: S202-7: ATTO 680
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePolyphosphoinositide phosphatase Antibody, 5-bisphosphate 5-phosphatase Antibody, Fig4 Antibody, KIAA0274 Antibody, Sac3 Antibody, Phosphatidylinositol 3 Antibody, Phosphatidylinositol 3,5 bisphosphate 5 phosphatase Antibody, SAC domain containing protein 3 Antibody, SAC domain-containing protein 3, SAC3 Antibody
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Raised inMouse
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Antibody s targetFIG4 phosphatase
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Primary research fieldsNeuroscience, Neurodegeneration, Cell Signaling, Post-translational Modifications, Phosphorylation
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-468 was sufficient for detection of FIG4/SAC3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellEndosome , Endosome membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextFig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy.
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Released date20-Nov-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_598760.1.
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Gene number103199
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Protein numberQ91WF7
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIG4/SAC3 Monoclonal Antibody, Clone S202-7 (SMC-468). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FIG4/SAC3 Monoclonal Antibody (SMC-468) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus, Endosome, Endosome membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FIG4/SAC3 Antibody (D) Composite. Western Blot analysis of Mouse Brain Membrane showing detection of ~100 kDa FIG4/SAC3 protein using Mouse Anti-FIG4/SAC3 Monoclonal Antibody, Clone S202-7 (SMC-468). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FIG4/SAC3 Monoclonal Antibody (SMC-468) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-FIG4/SAC3 Antibody [S202-7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-468) Mouse Anti-FIG4/SAC3 Antibody [S202-7] used in Western Blot (WB) on Mouse Brain Membrane (SMC-468)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolFIG4, PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, MCM3AP, PIRC30
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Short nameMouse Anti-Mouse FIG4/SAC3 Monoclonal IgG1, Clone S202-7
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 680
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse FIG4/SAC3 monoclonal IgG1, clonality S202-7
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Alternative techniquemurine, antibodies
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Clone nameS202-7
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Gene info
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Identity
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Gene
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Long gene nameFIG4 phosphoinositide 5-phosphatase
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Synonyms gene
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Synonyms gene name
- KIAA0274
- FIG4 homolog (S. cerevisiae)
- FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphoinositide phosphatases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 34
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 33
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 32
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-7
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR15-7 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR15-7
- immunoglobulin heavy variable 3/OR15-7 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 7
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameminichromosome maintenance complex component 3 associated protein
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Synonyms gene name
- minichromosome maintenance deficient (S. cerevisiae) 3-associated protein
- MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lysine acetyltransferases
- Transcription and export complex 2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 30
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data