Mouse Anti-Mouse FIG4/SAC3 Monoclonal IgG1, Clone S202-7

• Catalog number
  SMC-468D-A488
• Price
  Please ask
• Size
  100 µg

• Clone
  S202-7
• Immunogen
  Fusion protein amino acids 688-907 (C-terminus) of mouse Sac3. Rat: 95% identity (203/220 amino acids identical). Human: 91% identity (201/220 amino acids identical)
• Antibody s full description
  Mouse Anti-Mouse FIG4 phosphatase Monoclonal IgG1 Antibody, Clone: S202-7: ATTO 488
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  Polyphosphoinositide phosphatase Antibody, 5-bisphosphate 5-phosphatase Antibody, Fig4 Antibody, KIAA0274 Antibody, Sac3 Antibody, Phosphatidylinositol 3 Antibody, Phosphatidylinositol 3,5 bisphosphate 5 phosphatase Antibody, SAC domain containing protein 3 Antibody, SAC domain-containing protein 3, SAC3 Antibody
• Raised in
  Mouse
• Antibody s target
  FIG4 phosphatase
• Primary research fields
  Neuroscience, Neurodegeneration, Cell Signaling, Post-translational Modifications, Phosphorylation
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~100kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-468 was sufficient for detection of FIG4/SAC3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Endosome , Endosome membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy.
• Released date
  20-Nov-2013
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_598760.1.
• Gene number
  103199
• Protein number
  Q91WF7
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIG4/SAC3 Monoclonal Antibody, Clone S202-7 (SMC-468). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FIG4/SAC3 Monoclonal Antibody (SMC-468) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus, Endosome, Endosome membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FIG4/SAC3 Antibody (D) Composite. Western Blot analysis of Mouse Brain Membrane showing detection of ~100 kDa FIG4/SAC3 protein using Mouse Anti-FIG4/SAC3 Monoclonal Antibody, Clone S202-7 (SMC-468). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FIG4/SAC3 Monoclonal Antibody (SMC-468) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-FIG4/SAC3 Antibody [S202-7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-468) Mouse Anti-FIG4/SAC3 Antibody [S202-7] used in Western Blot (WB) on Mouse Brain Membrane (SMC-468)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  FIG4/SAC3   Monoclonal   IgG1   S202-7  
• Gene symbol
  FIG4, PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, MCM3AP, PIRC30
• Short name
  Mouse Anti-Mouse FIG4/SAC3 Monoclonal IgG1, Clone S202-7
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 488
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse FIG4/SAC3 monoclonal IgG1, clonality S202-7
• Alternative technique
  murine, antibodies
• Clone name
  S202-7

Gene info

• Identity
  HGNC:16873
• Gene
  FIG4
• Long gene name
  FIG4 phosphoinositide 5-phosphatase
• Synonyms gene
  • KIAA0274
• Synonyms gene name
  • KIAA0274
  • FIG4 homolog (S. cerevisiae)
  • FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae)
• Synonyms
  • SAC3
  • hSac3
  • dJ249I4.1
  • ALS11
  • CMT4J
• GenBank acession
  • D87464
• Locus
  6q21
• Discovery year
  2003-11-26
• Entrez gene record
  9896
• Pubmed identfication
  • 9039502
  • 11274189
  • 17572665
• RefSeq identity
  • NM_014845
• Classification
  • Phosphoinositide phosphatases
• VEGA ID
  OTTHUMG00000015352
• Locus Specific Databases
  • LRG_241

Gene info

• Identity
  HGNC:37538
• Gene
  PIRC34
• Long gene name
  piwi-interacting RNA cluster 34
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313890
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37537
• Gene
  PIRC33
• Long gene name
  piwi-interacting RNA cluster 33
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313854
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37536
• Gene
  PIRC32
• Long gene name
  piwi-interacting RNA cluster 32
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313811
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32995
• Gene
  SNORD114-7
• Long gene name
  small nucleolar RNA, C/D box 114-7
• Synonyms
  • 14q(II-7)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767583
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003199.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5633
• Gene
  IGHV3OR15-7
• Long gene name
  immunoglobulin heavy variable 3/OR15-7 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR15-7
  • immunoglobulin heavy variable 3/OR15-7 pseudogene
• Synonyms
  • IGHV3/OR15-7
• GenBank acession
  • Z29597
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28318
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000171835

Gene info

• Identity
  HGNC:33026
• Gene
  SNORD115-7
• Long gene name
  small nucleolar RNA, C/D box 115-7
• Synonyms
  • HBII-52-7
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033444
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003299.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:34284
• Gene
  RNU6-7
• Long gene name
  RNA, U6 small nuclear 7
• Synonyms
  • U6-7
• Locus
  14q12
• Discovery year
  2008-06-10
• Entrez gene record
  101954275
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_104084
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:6946
• Gene
  MCM3AP
• Long gene name
  minichromosome maintenance complex component 3 associated protein
• Synonyms gene name
  • minichromosome maintenance deficient (S. cerevisiae) 3-associated protein
  • MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein
• Synonyms
  • Map80
  • KIAA0572
  • GANP
  • SAC3
• Synonyms name
  • germinal-centre associated nuclear protein
• GenBank acession
  • AB005543
• Locus
  21q22.3
• Discovery year
  1999-03-04
• Entrez gene record
  8888
• Pubmed identfication
  • 9712829
  • 16914116
  • 21195085
• RefSeq identity
  • NM_003906
• Classification
  • Lysine acetyltransferases
  • Transcription and export complex 2
• VEGA ID
  OTTHUMG00000090630

Gene info

• Identity
  HGNC:37534
• Gene
  PIRC30
• Long gene name
  piwi-interacting RNA cluster 30
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313853
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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