This antibody has been succesfully tested for use in following methods: Western blot, Immunoprecipitation
Target of this antibody: XLF
Formulation of this antibody: 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Alternative names of antibody target: XRCC4-like Factor, Cernunnos, FLJ12610
Recommended usage: Western blotting (1:500 – 1:2500) and Immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the XLF of human origin. Reactivity to other species has not been tested.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide of human XLF
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: XLF (also called Cernunnos) interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated downregulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. XLF is homologous to the yeast non-homologous end-joining factor Nej1.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps