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Description
Simplest High-Throughput Assay To Screen Thioredoxin 1 Inhibitors
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Summary
• Detection method-Fluorescence (Ex/Em: 490/525 nm) • Applications- Screening/studying/characterizing Thioredoxin 1 inhibitors
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Detection Method
Fluorescence (Ex/Em: 490/525 nm)
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Species Reactivity
Human
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Applications
Screening/studying/characterizing Thioredoxin 1 inhibitors
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Features Benefits
• Rapid, simple & sensitive • Convenient & HTS • Includes inhibitor control
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
The human Thioredoxin 1 (Trx1) is a key enzyme that protects cells from oxidative stress by reducing disulfides and methionine sulfoxide groups in oxidized proteins. It also regulates DNA repair and apoptosis. In mammals, Trx1 is necessary for growth and development. However, an aberrant elevated expression of Trx1 is known to promote lung, cervical, pancreatic, colorectal, and breast cancers. In BioVision’s Trx1 Inhibitor Screening Kit, Trx1 reduces quenched di-FITC-labeled oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched reduced glutathione. In the presence of a Trx1 inhibitor (control inhibitor included), the reaction is impeded resulting in lower or no signal. The assay is high-throughput adaptable and can be completed in less than 1 hr
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
