Target of this antibody:
Alternative names of antibody target:
HD10 , Histone deacetylase 10
HDAC family are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. These results sμggest that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide near the N-terminus of human HDAC-10
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml), However, the optimal concentrations should be determined individually. The antibody recognizes 74 kDa HDAC-10 of human, mouse, and rat origins. A 55 kDa cleavage fragment can also be detected in mouse and rat tissue lysates.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.