pEGFP-N1-Flag-ASH1 Plasmid
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Catalog numberPVTB50076-2a
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Price382.49 USD
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Size2 ug
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PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbolLINC01109, SPANXN1, ELAVL2, ASCL1, ASH1L
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Short namepEGFP-N1- -ASH1 Plasmid
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Techniqueplasmid, plasmids in 1
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LabelFlag
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Alternative namepEGFP-N1-Flag-ASH1 Plasmid
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Alternative techniqueplasmids
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Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1109
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2013-10-11
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSPANX family member N1
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Synonyms gene name
- SPANX family, member N1
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Synonyms
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Synonyms name
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Locus
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Discovery year2006-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SPANX family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameELAV like RNA binding protein 2
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Synonyms gene name
- ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2
- ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
- ELAV like neuron-specific RNA binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameachaete-scute family bHLH transcription factor 1
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Synonyms gene name
- achaete-scute complex (Drosophila) homolog-like 1
- achaete-scute complex-like 1 (Drosophila)
- achaete-scute complex homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-09
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Entrez gene record
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Pubmed identfication
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameASH1 like histone lysine methyltransferase
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Synonyms gene name
- ash1 (absent, small, or homeotic)-like (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Bromodomain containing
- PHD finger proteins
- SET domain containing
- Lysine methyltransferases
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VEGA ID
