Target of this antibody:
Alternative names of antibody target:
p44-MAPK , p44erk1 , MGC20180 , MAPK3 , P44MAPK , HS44KDAP , ERK-1 , PRKM3 , HUMKER1A , ERT2
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs throμgh phosphorylation of threonine and tyrosine at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide from human p44 MAP kinase
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p44/42 MAP kinase (Erk1/Erk2) of human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.