TFF-3, human recombinant

• Catalog number
  4746-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Trefoil factor 3
• Alternative_names
  Trefoil factor 3 (Intestinal trefoil factor) (hITF) (Polypeptide P1.B) (hP1.B)
• Description
  A protein which protects and stabilizes the gastric mucus layer
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Purity
  ≥97%
• Biological activity
  Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.
• Results
  1.0-10.0 ng/ml.
• Molecular Weight
  13.2 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered white powder lyophilized from a solution containing PBS pH 7.4
• Reconstitution Instructions
  . Reconstitute in sterile 18 MΩ-cm H₂O at not less than 100 µg/ml. Reconstituted TFF3 should be stored in working aliquots at -20°C.
• Background Information
  Proteins of the TFF family are characterized by having at least 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, through an EGFR-dependent pathway.TFF-3 is normally expressed in hepatocellular carcinoma and its overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved disulfide bonds and has MW = 13.2 kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
• Amino acid sequence
  EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TFF-3  
• Gene symbol
  TFF3, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3, MIR941-3
• Short name
  TFF-3, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  TFF-3, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11757
• Gene
  TFF3
• Long gene name
  trefoil factor 3
• Synonyms gene name
  • trefoil factor 3 (intestinal)
• Synonyms
  • HITF
  • ITF
• GenBank acession
  • AF432265
• Locus
  21q22.3
• Discovery year
  1995-11-22
• Entrez gene record
  7033
• Pubmed identfication
  • 7718582
  • 9043862
• RefSeq identity
  • NM_003226
• VEGA ID
  OTTHUMG00000086798

Gene info

• Identity
  HGNC:35295
• Gene
  MIR1302-3
• Long gene name
  microRNA 1302-3
• Synonyms gene
  • MIRN1302-3
• Synonyms
  • hsa-mir-1302-3
• Locus
  2q14.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302128
• RefSeq identity
  • NR_031632
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:33675
• Gene
  MIR509-3
• Long gene name
  microRNA 509-3
• Synonyms gene
  • MIRN509-3
• Synonyms
  • hsa-mir-509-3
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126337
• RefSeq identity
  • NR_030629
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:37518
• Gene
  PIRC14
• Long gene name
  piwi-interacting RNA cluster 14
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313784
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37516
• Gene
  PIRC12
• Long gene name
  piwi-interacting RNA cluster 12
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313808
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:31646
• Gene
  MIR9-3
• Long gene name
  microRNA 9-3
• Synonyms gene
  • MIRN9-3
• Synonyms
  • hsa-mir-9-3
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407051
• RefSeq identity
  • NR_029692
• Classification
  • MicroRNA MIR9 family

Gene info

• Identity
  HGNC:33686
• Gene
  MIR941-3
• Long gene name
  microRNA 941-3
• Synonyms gene
  • MIRN941-3
• Synonyms
  • hsa-mir-941-3
• Locus
  20q13.33
• Discovery year
  2007-10-24
• Entrez gene record
  100126352
• RefSeq identity
  • NR_030639
• Classification
  • MicroRNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products