Trefoil factor 3
Trefoil factor 3 (Intestinal trefoil factor) (hITF) (Polypeptide P1.B) (hP1.B)
A protein which protects and stabilizes the gastric mucus layer
Purity by SDS-PAGE
Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.
Physical form description
Sterile filtered white powder lyophilized from a solution containing PBS pH 7.4
. Reconstitute in sterile 18 MΩ-cm H₂O at not less than 100 µg/ml. Reconstituted TFF3 should be stored in working aliquots at -20°C.
Proteins of the TFF family are characterized by having at least 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, through an EGFR-dependent pathway.TFF-3 is normally expressed in hepatocellular carcinoma and its overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved disulfide bonds and has MW = 13.2 kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
Amino acid sequence
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
TFF-3, H. sapiens recombinant