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TFF 3 human recombinant

TFF 3 human recombinant is available 3 times from Biovision labs

4746-100 | TFF-3, human recombinant size: 100 ug | 750.28 USD

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4746-20 | TFF-3, human recombinant size: 20 ug | 280.85 USD

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4746-1000 | TFF-3, human recombinant size: 1 mg | 4,638.99 USD

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Synonyms Trefoil factor 3
Alternative_names Trefoil factor 3 (Intestinal trefoil factor) (hITF) (Polypeptide P1.B) (hP1.B)
Description A protein which protects and stabilizes the gastric mucus layer
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥97%
Assay SDS-PAGE
Purity ≥97%
Biological activity Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.
Results 1.0-10.0 ng/ml.
Molecular Weight 13.2 kDa
Storage Temp. -20°C
Shipping Blue ice
Shelf Life 12 months
Appearance Lyophilized protein
Physical form description Sterile filtered white powder lyophilized from a solution containing PBS pH 7.4
Reconstitution Instructions . Reconstitute in sterile 18 MΩ-cm H₂O at not less than 100 µg/ml. Reconstituted TFF3 should be stored in working aliquots at -20°C.
Background Information Proteins of the TFF family are characterized by having at least 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, through an EGFR-dependent pathway.TFF-3 is normally expressed in hepatocellular carcinoma and its overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved disulfide bonds and has MW = 13.2 kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
Amino acid sequence EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetTFF-3
Short name TFF-3, recombinant
Technique Recombinant
Species Human
Alternative name TFF-3, H. sapiens recombinant
Alternative technique rec
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