BioSim™ Vedolizumab (Entyvio®)(Human) ELISA Kit

• Catalog number
  E4697-100
• Price
  Please ask
• Size
  96 assays

• Description
  This ELISA kit is used for in vitro quantitative determination of Vedolizumab.
• Summary
  Vedolizumab is a recombinant humanized IgG1 monoclonal antibody directed against the human lymphocyte α4β7 integrin, a key mediator of gastrointestinal inflammation. It is used in the treatment of moderate to severe active ulcerative colitis and Crohn's disease for patients who have had an inadequate response with, lost response to, or were intolerant to inhibitors of tumor necrosis factor-alpha (TNF-alpha) or other conventional therapies. BioSim™ Vedolizumab ELISA kit is a Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Vedolizumab (Cyramza®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to Vedolizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Vedolizumab in the sample or standard.
• Detection Method
  colorimetric
• Species Reactivity
  Human
• Applications
  Sensitivity: 30 ng/ml,Recovery rate: 85 – 115% with normal human serum samples with known concentrations,Detection Range: 30 - 1000 ng/ml,This ELISA kit is used for in vitro quantitative determination of Vedolizumab,Cross Reactivity: Except for Vedolizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
• Sample Type
  Plasma,Serum
• Features Benefits
  Detection Range: 30 - 1000 ng/ml,Cross Reactivity: Except for Vedolizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.,Sensitivity: 30 ng/ml,This ELISA kit is used for in vitro quantitative determination of Vedolizumab
• Storage Conditions
  4°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Vedolizumab is a recombinant humanized IgG1 monoclonal antibody directed against the human lymphocyte α4β7 integrin, a key mediator of gastrointestinal inflammation. It is used in the treatment of moderate to severe active ulcerative colitis and Crohn's disease for patients who have had an inadequate response with, lost response to, or were intolerant to inhibitors of tumor necrosis factor-alpha (TNF-alpha) or other conventional therapies. BioSim™ Vedolizumab ELISA kit is a Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Vedolizumab (Cyramza®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to Vedolizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Vedolizumab in the sample or standard.
• Data Sheet Link
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  BioSim™   Vedolizumab   Entyvio®  
• Short name
  BioSim™ Vedolizumab (Entyvio®)( ) ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Humans
• Alternative name
  BioSim™ Vedolizumab (Entyvio®)(H. sapiens) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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