Cas9 Monoclonal Antibody

• Catalog number
  A1069-50
• Price
  Please ask
• Size
  50 µg

• Antibody Target
  Cas9
• Target Alternative Name
  CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9
• Antibody Type
  Monoclonal
• Clone
  7A9
• Immunogen Sequence
  N-terminal fragment of Streptococcus pyogenes Cas9.
• Accession
  Q99ZW2
• Gene Id
  901176
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  PBS containing 0.02% sodium azide.
• Purification
  Affinity purified using protein G
• Application
  IF, IP, WB
• Positive Control
  IF, IP, WB
• Application And Usages
  Immunofluorescence: 1:500-1:2,000; Immunoprecipitation: 1:20; Western Blot: 1:1,000
• Description
  Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Cas9   Monoclonal  
• Short name
  Cas9 Monoclonal Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Isotype
  Mouse IgG1κ
• Alternative name
  Cas9 monoclonal (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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