Mitochondrial DNA Isolation Kit
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Catalog number
K280-50
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Price
Please ask
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Size
50 assays
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Description
Isolates mt. DNA, no genomic DNA contamination
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Summary
• Species reactivity- Mammalian • Applications- Enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.
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Sample Type
Cell cultures (adherent and suspension), fresh and frozen tissues
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Features Benefits
• Simple procedure; takes less than 2 hours • Fast and convenient • The kit provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction. Mitochondrial DNA are then isolated using reagents included in the kit
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Mitochondria are semiautonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA. The Mitochondrial DNA Extraction Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Mitochondrial DNA Isolation Kit
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Technique
dna
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Alternative name
Mitochondrial Desoxyribonucleic acid purification reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
mitochondrial
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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