Goat anti Rat IgG2b (subclass specific), conjugated with Biotin

Products_type

Secondary Antibody
Products_short_name

[Goat anti Rat IgG2b (subclass specific)]
Clonality

Polyclonal
Reactivity

Rat
Specificity

Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG2b, subclass specific.
Storage_stability

Store at 4 degree C.
Tested_application

ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Latin name

Capra aegagrus hircus, Rattus norvegicus
About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Gene target

IgG2b subclass specific conjugated with
Short name

Goat anti IgG2b (subclass specific), conjugated with
Technique

anti, Goat, antibody to, antibody Conjugates, goats
Host

Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
Isotype

IgG2b
Label

Biotin
Species

Rat, Goats, Rats
Alternative name

caprine antibody to Rat IgG2b (subclass specific), coupled including biotinilated
Alternative technique

antibodies, caprine

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data