anti-IL-10 Antibody

• Catalog number
  XP-5162
• Price
  Please ask
• Size
  0.1 mg

• Gene info
  Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
• Gene names
  CSIF, Il-10, Interleukin-10, Cytokine synthesis inhibitory factor, IL-10
• Antibodie s label
  unlabelled serum
• Purity
  Anti-mIL-10 specific antibody was purified by affinity chromatography employing immobilized mIL-10 matrix.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-10 (murine IL-10).
• Immuno application
  ELISA, Western Blot, NELISAut
• Antibody advice
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (2.5 ng/mL), a concentration of 0.012- 0.014 ug/mL of this antibody is required. ELISA:To detect mIL-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-10. SandwichTo detect mIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-10 (XP-5162Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10.Western Blot:To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Keep at
  IL-10 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
• Works with
  Mouse
• Omim nr
  408360145
• Ncbi nr
  P18893
• Vial with antibody
  Lyophilized
• NCBI GENE nr
  16153
• GENE symbol
  Il10
• Antigene name
  interleukin 10
• Source
  Mus musculus
• Protein nr
  P18893
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  IL-10  
• Gene symbol
  IL10, SNORD115-10, PIRC49, SNORD116-10, IL36G, IL25, IGKV2OR2-10
• Short name
  anti-IL-10 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toIL-10 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:5962
• Gene
  IL10
• Long gene name
  interleukin 10
• Synonyms
  • CSIF
  • TGIF
  • IL10A
  • IL-10
• Synonyms name
  • cytokine synthesis inhibitory factor
  • T-cell growth inhibitory factor
• GenBank acession
  • M57627
• Locus
  1q32.1
• Discovery year
  1991-10-31
• Entrez gene record
  3586
• Pubmed identfication
  • 9162098
• RefSeq identity
  • NM_000572
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000036386
• Locus Specific Databases
  • LRG_1230

Gene info

• Identity
  HGNC:33029
• Gene
  SNORD115-10
• Long gene name
  small nucleolar RNA, C/D box 115-10
• Synonyms
  • HBII-52-10
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033447
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003302.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:37553
• Gene
  PIRC49
• Long gene name
  piwi-interacting RNA cluster 49
• Locus
  10
• Discovery year
  2009-11-05
• Entrez gene record
  100313929
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:33076
• Gene
  SNORD116-10
• Long gene name
  small nucleolar RNA, C/D box 116-10
• Synonyms
  • HBII-85-10
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033422
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003325
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:15741
• Gene
  IL36G
• Long gene name
  interleukin 36 gamma
• Synonyms gene
  • IL1F9
• Synonyms gene name
  • interleukin 1 family, member 9
• Synonyms
  • IL-1H1
  • IL-1RP2
  • IL-1F9
  • IL1H1
  • IL1E
• Synonyms name
  • interleukin-1 homolog 1
  • interleukin 1-related protein 2
  • interleukin-1 epsilon
• GenBank acession
  • AF200492
• Locus
  2q14.1
• Discovery year
  2002-08-02
• Entrez gene record
  56300
• Pubmed identfication
  • 10860666
  • 10744718
  • 11991722
  • 11991723
• RefSeq identity
  • NM_019618
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000131336

Gene info

• Identity
  HGNC:13765
• Gene
  IL25
• Long gene name
  interleukin 25
• Synonyms gene
  • IL17E
• Synonyms gene name
  • interleukin 17E
• Synonyms
  • IL-25
  • IL-17E
• GenBank acession
  • AF305200
• Locus
  14q11.2
• Discovery year
  2001-01-26
• Pubmed identfication
  • 11058597
  • 11754819
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000028749

Gene info

• Identity
  HGNC:5806
• Gene
  IGKV2OR2-10
• Long gene name
  immunoglobulin kappa variable 2/OR2-10 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-10
  • immunoglobulin kappa variable 2/OR2-10 pseudogene
• Synonyms
  • IGKV2/OR2-10
• GenBank acession
  • X51886
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28855
• RefSeq identity
  • NG_011661
• Classification
  • Immunoglobulin kappa (IGK) orphons

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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