pIRES2-EGFP-Flag-ELP3-HA Plasmid
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Catalog number
PVTB00264-2g
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Price
381.42 USD
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Size
2 ug
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Conjugate
The Green Fluorescent Protein (eGFP) of pIRES2- - -ELP3-HA Plasmid can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
ELP3, MYO1G, KRT31, KRT32, KRT33B, KRT35, KRT34, ARHGAP45, KRT33A, AKAP13
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Short name
pIRES2- - -ELP3-HA Plasmid
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Technique
plasmid, plasmids in 1
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Label
Flag
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Alternative name
pIRES2-EGFP-Flag-ELP3-HA Plasmid
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Alternative technique
plasmids
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Gene info
Gene info
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Identity
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Gene
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Long gene name
myosin IG
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 31
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 1
- keratin 31, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 32
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 2
- keratin 32, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 33B
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 3B
- keratin 33B, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 35
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 5
- keratin 35, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 34
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 4
- keratin 34, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
keratin 33A
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Synonyms gene
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Synonyms gene name
- keratin, hair, acidic, 3A
- keratin 33A, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2004-10-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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