Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
• Detection method: Fluorescence (Ex/Em 380/461 nm) • Species reactivity: Mammalian • Applications: The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.
• Simple two-step procedure; takes only 2 hours • Fast and convenient • The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione.
Kit's other name
• Cell Lysis Buffer • Monochlorobimane • GST Reagent • GSH Standard (1 mg; MW: 307)
Maximum time can be stored
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Biovision supplies other types of Assays as 1.
Glutathione Fluorometric Assay Kit
GSH fluorescence test reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832