Glutathione Fluorometric Assay Kit

• Catalog number
  K251-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple assay to detect total glutathione
• Summary
  • Detection method- Fluorescence (Ex/Em 380/461 nm) • Species reactivity- Mammalian • Applications- The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple two-step procedure; takes only 2 hours • Fast and convenient • The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Glutathione   Fluorometric   Kit  
• Short name
  Glutathione Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  GSH fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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