Mouse Anti-Mouse REEP1/2 Monoclonal IgG1, Clone S326D-29
-
Catalog numberSMC-482D-RPE
-
PricePlease ask
-
Size100 µg
-
-
CloneS326D-29
-
ImmunogenFusion protein amino acids 111-254 (Cytoplasmic C-terminus) of mouse REEP2.
-
Antibody s full descriptionMouse Anti-Mouse REEP1/2 Monoclonal IgG1 Antibody, Clone: S326D-29: RPE
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameReceptor expression-enhancing protein 2 Antibody, C5orf19 Antibody, SGC32445 Antibody, LOC682105 Antibody
-
Raised inMouse
-
Antibody s targetREEP1/2
-
Primary research fieldsNeuroscience, Neurodegeneration, Spastic Paraplegia, Cell Structure, Membrane Markers
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration0.5 mg/ml
-
Antibody s specificityDetects ~30kDa. Cross-reacts with REEP1.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysisA 1:100 dilution of SMC-482 was sufficient for detection of REEP1/2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellMitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane
-
Tissue specificitySee included datasheet or contact our support service
-
Bibliography1. Hurt C.M., et al. (2014) Brain Res. 1545: 12-22.
-
Released date10-Mar-2015
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_001191843.1
-
Gene number225362
-
Protein numberQ8VCD6
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP1/2 Monoclonal Antibody, Clone S326D-29 (SMC-482). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1/2 Monoclonal Antibody (SMC-482) at 1:50 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP1/2 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~30 kDa REEP1-2 protein using Mouse Anti-REEP1-2 Monoclonal Antibody, Clone S326D-29 (SMC-482). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1-2 Monoclonal Antibody (SMC-482) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~30 kDa. Other Band(s): ~22 kDa. Mouse Anti-REEP1/2 Antibody [S326D-29] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-482) Mouse Anti-REEP1-2 Antibody [S326D-29] used in Western Blot (WB) on Rat Brain (SMC-482)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolSNORD114-29, SNORD115-29, SNORD116-29, IGHV3-29, IGLV3-29, IGKV2-29, ERVK-29, IGKV2D-29, ERVW-29, RNVU1-29
-
Short nameMouse Anti-Mouse REEP1/2 Monoclonal IgG1, Clone S326D-29
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelRPE
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toMouse REEP1/2 monoclonal IgG1, clonality S326D-29
-
Alternative techniquemurine, antibodies
-
Clone nameS326D-29
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-29
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-29
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-29
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-29 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 3-29
- immunoglobulin heavy variable 3-29 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 3-29 (pseudogene)
-
Synonyms gene name
- immunoglobulin lambda variable 3-29
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2-29
-
Synonyms gene name
- immunoglobulin kappa variable 2-29 (gene/pseudogene)
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group K member 29
-
Synonyms gene name
- endogenous retrovirus group K, member 29
-
GenBank acession
-
Locus
-
Discovery year2014-01-03
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2D-29
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group W member 29
-
Synonyms gene name
- endogenous retrovirus group W, member 29
-
GenBank acession
-
Locus
-
Discovery year2013-10-21
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, variant U1 small nuclear 29
-
Locus
-
Discovery year2019-07-26
-
Classification
- Variant U1 small nuclear RNAs
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data