p53 (C-terminus) monoclonal antibody

Alternative Name

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
Immunogen

Synthetic peptide, corresponding to the C-terminus of Human p53.
Swiss Prot

P04637
Reactivity

Human,Mouse
Applications

WB
Application_all

WB: 1:1000
Product

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

This antibody detects endogenous levels of p53 (C-terminus) and does not cross-react with related proteins
Usage

Western blot detection of P53 in K562 cell lysates using P53 antibody (1:1000 diluted) .
BiowMW

Predicted band size:53KDa Observed band size:53KDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translation

anticorps

Gene target

p53 monoclonal
Short name

p53 ( ) monoclonal antibody
Technique

Antibody, antibodies against human proteins, antibodies for, antibodies directed against c-terminus peptides can be suited for IHC because in the quaternary structure the c-terminus of this protein is often at the outside of the cell membrane. Monoclonals or monoclonal antibodies
Host

Mouse
Alternative name

p53 (C-terminus) monoclonal (antibody to-)
Alternative technique

antibodies

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

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