CCL4 Antibody, FITC Conjugated
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Catalog number
bs-2475R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
CCL4 Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
CCL4
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Specificity
This is a highly specific antibody against CCL4.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic peptide derived from mouse CCL4
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Gene ID number
6351
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
MIP-1 Beta; Macrophage Inflammatory Protein 1 beta; MIP-1 Beta; MIP-1β; Secreted protein G 26; ACT 2; ACT2; AT744.1; CC chemokine ligand 4; CCL4; ccl4l 1; CCL4L1; Chemokine CC Mot Ligand 4; G 26 T lymphocyte secreted protein; HC21; LAG 1; LAG1; Lymphocyte activation gene 1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1 beta; MGC104418; MGC126025; MGC126026; MIP1 beta; MIP1B; MIP1B1; PAT 744; Protein H400; SCYA2; SCYA4; Secreted protein G26; SIS gamma; Small inducible cytokine A4; T cell activation protein 2; CCL4_MOUSE;
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
CCL4
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Short name
Anti-CCL4
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-CCL4 PAb FITC
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Alternative technique
antibodies, fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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