Phosphogluconate dehydrogenase, human recombinant

• Catalog number
  P1051-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  6PGD, PGD
• Alternative_names
  6PGD, PGD
• Description
  An enzyme involved in the pentose phosphate shunt, major source of NADPH.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  > 90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of ß-NADP.
• Biological activity
  Specific activity is >10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of ß-NADP.
• Results
  >10 units/mg
• Unit Definition
  One unit is the amount of enzyme that oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of ß-NADP.
• Molecular Weight
  53.3 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol
• Background Information
  PGD (Phosphogluconate dehydrogenase), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Phosphogluconate   dehydrogenase  
• Gene symbol
  PGDP1, PGD, PGDP2
• Short name
  Phosphogluconate dehydrogenase, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Phosphogluconate dehydrogenase, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8892
• Gene
  PGDP1
• Long gene name
  phosphogluconate dehydrogenase pseudogene 1
• Synonyms gene
  • PGDL1
• Synonyms gene name
  • phosphogluconate dehydrogenase-like 1
• Locus
  18q12.1
• Discovery year
  1989-05-25
• Entrez gene record
  342705
• RefSeq identity
  • NG_022489
• VEGA ID
  OTTHUMG00000179618

Gene info

• Identity
  HGNC:8891
• Gene
  PGD
• Long gene name
  phosphogluconate dehydrogenase
• GenBank acession
  • BC000368
• Locus
  1p36.22
• Discovery year
  2001-06-22
• Entrez gene record
  5226
• RefSeq identity
  • NM_002631
• VEGA ID
  OTTHUMG00000001905

Gene info

• Identity
  HGNC:39144
• Gene
  PGDP2
• Long gene name
  phosphogluconate dehydrogenase pseudogene 2
• Locus
  12q21.2
• Discovery year
  2010-10-21
• Entrez gene record
  100129649
• RefSeq identity
  • NG_021727

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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