Brain Derived Neurotrophic Factor (BDNF) Polyclonal Antibody (Guinea pig), FITC

• Catalog number
  PAA011Gu01-100ul-FITC
• Price
  Please ask
• Size
  100ul

• Description
  A Rabbit polyclonal antibody against Guinea pig Brain Derived Neurotrophic Factor (BDNF). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Guinea pig; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: BDNF (Ser147~Leu249); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
• Conjugation
  Anti-FITC Antibody
• About
  Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• Group
  Polyclonals and antibodies
• French translation
  anticorps

• Gene target
  Brain   Derived   Neurotrophic   Factor   BDNF  
• Gene symbol
  BDNF, BDNF-AS
• Short name
  Brain Derived Neurotrophic Factor (BDNF) Polyclonal Antibody ( ), FITC
• Technique
  Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  FITC
• Species
  Guinea Pig, Guinea Pigs, Pigs and Guinea pigs
• Alternative name
  Brain Derived Neurotrophic Factor (brain-derived neurotrophic factor) polyclonal (antibody to-) (Guinea swine), fluorecein
• Alternative technique
  polyclonals, antibodies, fluorescine
• Alternative to gene target
  brain-derived neurotrophic factor, ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, growth factor activity, Extracellular, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701
• Tissue
  brain

Gene info

• Identity
  HGNC:1033
• Gene
  BDNF
• Long gene name
  brain derived neurotrophic factor
• Synonyms name
  • neurotrophin
• GenBank acession
  • AB038670
• Locus
  11p14.1
• Discovery year
  1991-01-15
• Entrez gene record
  627
• Pubmed identfication
  • 2236018
  • 1889806
  • 17942328
  • 17493809
• RefSeq identity
  • NM_170735
• Classification
  • Neurotrophins
• VEGA ID
  OTTHUMG00000178797

Gene info

• Identity
  HGNC:20608
• Gene
  BDNF-AS
• Long gene name
  BDNF antisense RNA
• Synonyms gene
  • BDNFOS
• Synonyms gene name
  • BDNF opposite strand (non-protein coding)
  • BDNF antisense RNA (non-protein coding)
• Synonyms
  • BT2A
  • BT2B
  • BT2C
  • BT2D
  • NCRNA00049
  • BDNF-AS1
  • BDNFAS
• Synonyms name
  • non-protein coding RNA 49
  • BDNF antisense RNA 1 (non-protein coding)
• GenBank acession
  • AY054391
• Locus
  11p14.1
• Discovery year
  2005-02-01
• Entrez gene record
  497258
• Pubmed identfication
  • 15666411
  • 22446693
  • 28777258
• RefSeq identity
  • NR_002832
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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