Recombinant Human SMAD Family Member 4/SMAD4/DPC4 Protein(C-6His)
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Catalog number
PKSH033066-10µg
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Price
Please ask
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Size
10µg
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Synonym
Mothers Against Decapentaplegic Homolog 4; MAD Homolog 4; Mothers Against DPP Homolog 4; Deletion Target in Pancreatic Carcinoma 4; SMAD Family Member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4
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Activity
NA
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Sequence
Met1-Asp552
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Fusion tag
C-6His
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Accession
Q13485
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Expressed Host
E.coli
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
＞85% as determined by reducing SDS-PAGE.
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Endotoxin
＜1.0 EU per µg as determined by LAL test.
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Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass
61.5 kDa
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AP Mol Mass
62 kDa
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Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl，pH8.0.
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Background
SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
SMAD4
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Short name
Recombinant SMAD Family Member 4/SMAD4/DPC4 Protein(C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens SMAD family Member 4/SMAD family member 4/DPC4 Protein(C-6His)
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Alternative technique
rec
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Alternative to gene target
SMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
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Gene info
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Identity
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Gene
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Long gene name
SMAD family member 4
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Synonyms gene
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Synonyms gene name
- MAD, mothers against decapentaplegic homolog 4 (Drosophila)
- SMAD, mothers against DPP homolog 4 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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