HA-tag (4G3) monoclonal antibody

Alternative Name

HA epitope tag; HA Tag; hemagglutinin
Immunogen

Synthetic peptide HA-tag.
Swiss Prot

N/A
Reactivity

All
Applications

WB IF IP
Application_all

WB: 1:1000~1:5000 IF: 1:1000 IP: 1:100
Product

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

HA-tag (4G3) mAb detects over-expressed proteins containing the HA epitope tag.
Usage

Western blot (WB) analysis of over-expressed HA-tagged protein in HEK293T cell lysate, the antibody dilutions are 1:1000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of cell lysate.
BiowMW

N/A
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translation

anticorps

Gene target

HA-tag 4G3 monoclonal
Gene symbol

MYO1G, KRT31, KRT32, KRT33B, KRT34, KRT35, ARHGAP45, KRT33A, AKAP13, PUM3
Short name

HA-tag (4G3) monoclonal antibody
Technique

Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
Host

Mouse
Label

tag
Alternative name

HA-detection labelled (4G3) monoclonal (antibody to-)
Alternative technique

antibodies

Identity

HGNC:13880
Gene

MYO1G
Long gene name

myosin IG
Synonyms
- HA-2
Synonyms name
- minor histocompatibility antigen HA-2
GenBank acession
- AF380932
Locus

7p13
Discovery year

2000-11-28
Entrez gene record

64005
Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000155821

Identity

HGNC:17102
Gene

ARHGAP45
Long gene name

Rho GTPase activating protein 45
Synonyms gene
- HMHA1
Synonyms gene name
- histocompatibility (minor) HA-1
Synonyms
- KIAA0223
- HA-1
GenBank acession
- D86976
Locus

19p13.3
Discovery year

2005-12-06
Entrez gene record

23526
Pubmed identfication
- 9820596
- 9039502
Classification
- Minor histocompatibility antigens
- AH/BAR family Rho GTPase activating proteins
- F-BAR domain containing

Identity

HGNC:371
Gene

AKAP13
Long gene name

A-kinase anchoring protein 13
Synonyms gene
- LBC
Synonyms gene name
- lymphoid blast crisis oncogene
- A kinase (PRKA) anchor protein 13
Synonyms
- Ht31
- BRX
- AKAP-Lbc
- c-lbc
- PROTO-LB
- HA-3
- ARHGEF13
GenBank acession
- M90360
Locus

15q25.3
Discovery year

1999-09-16
Entrez gene record

11214
Pubmed identfication
- 9627117
- 1860836
RefSeq identity
- NM_007200
Classification
- A-kinase anchoring proteins
- Dbl family Rho GEFs
- Minor histocompatibility antigens
- Pleckstrin homology domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000172205

Identity

HGNC:29676
Gene

PUM3
Long gene name

pumilio RNA binding family member 3
Synonyms gene
- KIAA0020
Synonyms gene name
- KIAA0020
Synonyms
- XTP5
- PEN
- PUF6
- hPUF-A
- HA-8
- Puf-A
Synonyms name
- penguin homolog (Drosophila)
- minor histocompatibility antigen HA-8
GenBank acession
- AL832239
Locus

9p24.2
Discovery year

2004-01-06
Entrez gene record

9933
Pubmed identfication
- 7584026
- 7584028
- 21266351
RefSeq identity
- NM_014878
Classification
- Pumilio homology domain containing
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000019450

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data