EHMT2 Antibody

#

• Catalog number
  6152-100
• Price:

  419.37 USD

  Ask for price
• Size
  100 μg

# #

• Antibody Target
  EHMT2
• Target Alternative Name
  BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG3
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Recombinant protein of human EHMT2
• Accession
  Q96KQ7
• Gene Id
  10919
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot, Immunohistochemistry
• Positive Control
  Western Blot: Various cell lysates
• Application And Usages
  Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
• Description
  G9a, also known as Euchromatic histone­lysine N­methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases. Recombinant G9a can mono­, di­ and tri­methylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9a­related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono­ and di­methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero­dimerization and complex stability. Wiz contains two CtBP co­repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co­repressor complex. In addition, G9a and GLP are components of other large transcriptional co­repressor complexes, such as those involving E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication. G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes. In addition, G9a facilitates increased global levels of di­methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocellular carcinoma.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

#

• Gene target
  EHMT2  
• Gene symbol
  EHMT2-AS1, EHMT2
• Short name
  EHMT2 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  EHMT2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:39751
• Gene
  EHMT2-AS1
• Long gene name
  EHMT2 and SLC44A4 antisense RNA 1
• Synonyms gene name
  • EHMT2 antisense RNA 1 (non-protein coding)
  • EHMT2 antisense RNA 1
• Locus
  6p21.33
• Discovery year
  2011-04-21
• Entrez gene record
  107986588
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000057535

Gene info

• Identity
  HGNC:14129
• Gene
  EHMT2
• Long gene name
  euchromatic histone lysine methyltransferase 2
• Synonyms gene
  • C6orf30
  • BAT8
• Synonyms gene name
  • chromosome 6 open reading frame 30
  • HLA-B associated transcript 8
• Synonyms
  • G9A
  • Em:AF134726.3
  • NG36/G9a
  • KMT1C
• GenBank acession
  • AF134726
• Locus
  6p21.33
• Discovery year
  2001-07-09
• Entrez gene record
  10919
• Pubmed identfication
  • 8457211
  • 11316813
• RefSeq identity
  • NM_006709
• Classification
  • SET domain containing
  • Lysine methyltransferases
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000031180

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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