pcDNA3.1-Flag-CASP8 Plasmid

• Catalog number
  PVTB00872-2a
• Price
  381.42 USD
• Size
  2 ug

• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

• Gene target
  pcDNA3   CASP8  
• Gene symbol
  CASP8
• Short name
  pcDNA3 1- -CASP8 Plasmid
• Technique
  plasmid, plasmids in 1
• Label
  Flag
• Alternative name
  pcDNA3.1-Flag-caspase 8, apoptosis-related cysteine peptidase Plasmid
• Alternative technique
  plasmids
• Alternative to gene target
  caspase 8, apoptosis-related cysteine peptidase, ALPS2B and CAP4 and Casp-8 and FLICE and MACH and MCH5, CASP8 and IDBG-78534 and ENSG00000064012 and 841, cysteine-type endopeptidase activity involved in apoptotic signaling pathway, nuclei, Casp8 and IDBG-157464 and ENSMUSG00000026029 and 12370, CASP8 and IDBG-643207 and ENSBTAG00000015718 and 507481

Gene info

• Identity
  HGNC:1509
• Gene
  CASP8
• Long gene name
  caspase 8
• Synonyms gene name
  • caspase 8, apoptosis-related cysteine protease
  • caspase 8, apoptosis-related cysteine peptidase
• Synonyms
  • MCH5
  • MACH
  • FLICE
  • Casp-8
• GenBank acession
  • U60520
• Locus
  2q33.1
• Discovery year
  1996-11-11
• Entrez gene record
  841
• Pubmed identfication
  • 8681376
  • 8681377
• RefSeq identity
  • NM_001228
• Classification
  • Caspases
  • Death effector domain containing
  • Death inducing signaling complex
  • Ripoptosome
• VEGA ID
  OTTHUMG00000132821
• Locus Specific Databases
  • CASP8base: Mutation registry for Caspase 8 deficiency
  • LRG_34

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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