Mouse anti TCF-1

• Catalog number
  X1072M
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).
• Antibody come from
  Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 102-269 from the human TCF1A splice variant protein fused to maltose binding protein (MBP)
• Other description
  Provided as 0.2µm sterile filtered solution in 0.1 M tris-glycine, pH 7.4, 0.150M NaCl, 30 % glycerol with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  7H3
• Antigen antibody binding interaction
  Mouse anti TCF-1 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, Immunohistochemistry (Frozen Sections)
• Antibody s suited for
  Detects multiple human TCF-1 isotypes by Western blot at 0.5 to 2 µg/ml. Detects bands at 26, 32, 38, 42 and 48 kDa in Jurkat cell lysates. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Staal, F.J., et al. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int. Immunol. 1999, 11, 317-323_x000B__x000B_2. Mayer, K., et al. Ectopic activation of lymphoid high mobility group-box transcription factor TCF-1 and overexpression in colorectal cancer cells. Int. J. Cancer 1997, 72, 625-630_x000B__x000B_3. Castrop, K., et al. The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-linkage lymphocytes. Blood 1995, 86, 3050-3059_x000B__x000B_4. Van de Wetering, M, et al. 'Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties.' Mol. Cell. Biol., 16: 745-52 (1996)._x000B__x000B_Product Specific References:_x000B__x000B_1. Lin, C-W, et al. 'CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.' Blood, 2005, 106, 3567-3574.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  TCF-1  
• Gene symbol
  TCF7, TCF7L2, HNF1A, HGF, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1
• Short name
  Mouse anti TCF-1
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to TCF-1
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:11639
• Gene
  TCF7
• Long gene name
  transcription factor 7
• Synonyms gene name
  • transcription factor 7 (T-cell specific, HMG-box)
• Synonyms
  • TCF-1
• Synonyms name
  • T-cell-factor-7
• GenBank acession
  • Z47362
• Locus
  5q31.1
• Discovery year
  1991-08-18
• Entrez gene record
  6932
• RefSeq identity
  • NM_201634
• Classification
  • TCF/LEF transcription factor family
  • Wnt enhanceosome complex
• VEGA ID
  OTTHUMG00000129124

Gene info

• Identity
  HGNC:11641
• Gene
  TCF7L2
• Long gene name
  transcription factor 7 like 2
• Synonyms gene
  • TCF4
• Synonyms gene name
  • transcription factor 7-like 2 (T-cell specific, HMG-box)
• Synonyms
  • TCF-4
• GenBank acession
  • X62871
• Locus
  10q25.2-q25.3
• Discovery year
  1998-01-20
• Entrez gene record
  6934
• Pubmed identfication
  • 1741298
• RefSeq identity
  • NM_030756
• Classification
  • TCF/LEF transcription factor family
  • Wnt enhanceosome complex
• VEGA ID
  OTTHUMG00000019070

Gene info

• Identity
  HGNC:11621
• Gene
  HNF1A
• Long gene name
  HNF1 homeobox A
• Synonyms gene
  • MODY3
  • TCF1
• Synonyms gene name
  • transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor
• Synonyms
  • HNF1
  • LFB1
  • HNF1α
• GenBank acession
  • M57732
• Locus
  12q24.31
• Discovery year
  1990-02-12
• Entrez gene record
  6927
• Pubmed identfication
  • 1535333
  • 7795649
• RefSeq identity
  • NM_000545
• Classification
  • HNF class homeoboxes
• VEGA ID
  OTTHUMG00000151015
• Locus Specific Databases
  • Global Variome shared LOVD
  • LRG_522

Gene info

• Identity
  HGNC:4893
• Gene
  HGF
• Long gene name
  hepatocyte growth factor
• Synonyms gene
  • DFNB39
• Synonyms gene name
  • deafness, autosomal recessive 39
  • hepatocyte growth factor (hepapoietin A; scatter factor)
• Synonyms
  • SF
  • F-TCF
  • HGFB
  • HPTA
• Synonyms name
  • hepatopoietin A
  • fibroblast-derived tumor cytotoxic factor
  • scatter factor
  • lung fibroblast-derived mitogen
• Locus
  7q21.11
• Discovery year
  1991-06-07
• Entrez gene record
  3082
• Pubmed identfication
  • 1837206
  • 19576567
• RefSeq identity
  • NM_000601
• VEGA ID
  OTTHUMG00000023804

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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