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Category
Primary Antibodies
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Long description
Recognizes a protein of 59kDa, which is identified as Fyn. p59fyn is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. This antibody shows no cross-reaction with other members of the Src protein kinase family. Its epitope spans between aa 26-75 of the unique region of p59fyn. The unique N-terminal domain of p59fyn interacts with the CD3 and z chains of the TcR. p59fyn can bind other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of fyn activity. p59fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with synthetic peptide of 31 amino acids from the C-terminus of human pS2 protein and mouse myeloma p3-NS1-Ag4-1 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
1S
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Antigen antibody binding interaction
Mouse anti Fyn Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Kinase Assay, Western Blot, Immunohistochemistry (Paraffin Sections)
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Antibody s suited for
This antibody can be used for immunohistochemistry on frozen and formalin/paraffin embedded tissues (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Masaiakowski P, et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res 10:7895-7903, 1982._x000B__x000B_2. Jeltsh JM, et al. Structure of human oestrogen-responsive gene pS2. Nucleic Acids Res 15:1401-1414, 1987._x000B__x000B_3. Mori K, et al. Complete primary structure of the oestrogen-responsive gene (pS2) product. J Biochem 107:73-76, 1990_x000B__x000B_4. Rio MC, et al. Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. Science 241:705-708, 1988._x000B__x000B_5. Rio MC, et al. Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesteroneeceptors and the oncogener ERB B2, Proc Natl Acad Sci USA 84:9243-9247, 1987._x000B__x000B_6. Molina R, et al. Estrogen-inducible pS2 protein: Tumor characteristics and clinical behavior in breast cancer. Proc Endocrine Soc 61(#148), 1990._x000B__x000B_7. Abbondanzo SL, et. al. Prognostic significance of immuno-cytochemically determined pS2 in axillary node-negative breast carcinoma. Breast Cancer Res Treat 16:182, 1990.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
FYN, FRK, FYB1
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Short name
Mouse anti Fyn
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Technique
Mouse, anti, antibody to, mouses
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Host
mouse
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Isotype
IgG1
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to FYN oncogene related to v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian), FGR, YES
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Alternative technique
murine, antibodies
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Alternative to gene target
FYN oncogene related to SRC, FGR, YES, p59-FYN and SLK and SYN, FYN and IDBG-95463 and ENSG00000010810 and 102724705,2534, transferase activity, nuclei, Fyn and IDBG-144629 and ENSMUSG00000019843 and 14360, FYN and IDBG-631420 and ENSBTAG00000011851 and 527263
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