HPRT1, human recombinant

• Catalog number
  P1092-50
• Price
  Please ask
• Size
  50 µg

• Synonyms
  Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT
• Alternative_names
  Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT
• Description
  Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate.
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
• Biological activity
  >15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
• Results
  >15 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
• Molecular Weight
  26.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol
• Background Information
  Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKGGYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HPRT1  
• Gene symbol
  HPRT1
• Short name
  HPRT1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  hypoxanthine phosphoribosyltransferase 1, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  hypoxanthine phosphoribosyltransferase 1, HGPRT and HPRT, HPRT1 and IDBG-86864 and ENSG00000165704 and 3251, guanine phosphoribosyltransferase activity, Cytoplasm, Hprt and IDBG-145409 and ENSMUSG00000025630 and 15452, LOC510369 and IDBG-640500 and ENSBTAG00000046220 and 510369

Gene info

• Identity
  HGNC:5157
• Gene
  HPRT1
• Long gene name
  hypoxanthine phosphoribosyltransferase 1
• Synonyms gene
  • HPRT
• Synonyms
  • HGPRT
• Synonyms name
  • Lesch-Nyhan syndrome
• GenBank acession
  • M26434
• Locus
  Xq26.2-q26.3
• Discovery year
  2001-06-22
• Entrez gene record
  3251
• Pubmed identfication
  • 12175903
• RefSeq identity
  • NM_000194
• VEGA ID
  OTTHUMG00000022452
• Locus Specific Databases
  • Hypoxanthine Guanine Phosphoribosyltransferase 1; Lech-Nyhan Syndrome

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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