MOUSE Anti-CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN:FITC Antibody
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Catalog number
GEN216751
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Price
Please ask
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Size
100ug
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Also known as
CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
HCMV37
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Host organism
Mouse (Mus musculus)
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Species reactivity
Viral; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN This item is specific for Cytomegalovirus glycoprotein B late neutralizing antigen._x000D_Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5). _x000D__x000D_Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of approximately 100kDa. _x000D__x000D_This item reacts with MRC-5 infected cells but not uninfected cells. This antibody has been found to be neutralizing.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. To cause (an acid or base) to undergo neutralization.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Short name
MOUSE Anti-CYTOMEGALOVIRUS gB LATE ANTIGEN:FITC Antibody
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Technique
Antibody, antigen, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antigenes, Fluorescein, mouses
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Host
mouse
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Label
FITC
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Species
Cytomegalovirus, Cytomegaloviruses, Mouses
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Alternative name
Anti-MOUSE ANTI CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN:FITC
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Alternative technique
antibodies, antigenes, murine, fluorescine
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Disease
Cytomegalovirus or human CMV
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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