Mouse Anti-Mouse Nav beta 2 Monoclonal IgG2B, Clone S395-68
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Catalog numberSMC-485D-PCP
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PricePlease ask
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Size100 µg
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CloneS395-68
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ImmunogenFusion protein amino acids 1-215 (full-length) of mouse NavBeta2
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Antibody s full descriptionMouse Anti-Mouse NaVbeta2 Monoclonal IgG2B Antibody, Clone: S395-68: PerCP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameSodium channel subunit beta-2 Antibody, Scn2b Antibody, UNQ326/PRO386 Antibody, Gm183 Antibody
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Raised inMouse
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Antibody s targetNaVbeta2
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Primary research fieldsNeuroscience, Ion Channels, Sodium Channels, Voltage-Gated Sodium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~40kDa. Does not cross-reat with NavBeta1, Navbeta3, or Navbeta4.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-485 was sufficient for detection of NavBeta2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextNavBeta2 or Scn2B, plays a crucial role in the assembly, expression and functional modulation of the heterotrimeric complex of the sodium channel. The subunit, beta-2 causes an increase in the plasma membrane surface area and in its folding into microvilli. It also interacts with TNR, playing a crucial role in clustering and regulation of activity of sodium channels at the nodes of Ranvier (1, 2). Mutations in this gene have been linked to Brugada syndrome and atrial fibrillation (3).
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Bibliography1. Genes and mapped phenotypes. (n.d.). Retrieved March 16, 2015, from more 2. Eubanks J., Srinivasan J., Dinulos M.B., Disteche C.M., Catterall W.A. (1997) Neuroreport. 8(12): 2775-2779. 3. Riuro H., et al. (2013) Hum Mutat. 34(7): 961-966.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001014761.1
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Gene number72821
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Protein numberQ56A07
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 2 Monoclonal Antibody, Clone S395-68 (SMC-485). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 2 Monoclonal Antibody (SMC-485) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Nav beta 2 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~40 kDa Nav-Beta-2 protein using Mouse Anti-Nav-Beta-2 Monoclonal Antibody, Clone S395-68 (SMC-485). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Nav-Beta-2 Monoclonal Antibody (SMC-485) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa. Mouse Anti-Nav beta 2 Antibody [S395-68] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-485) Mouse Anti-Nav-Beta-2 Antibody [S395-68] used in Western Blot (WB) on Rat Brain (SMC-485)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGLVI-68, IGHV1-68, LCMT1, RNU6-68P, CRTAC1, INTS11, DTD1
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Short nameMouse Anti-Mouse Nav beta 2 Monoclonal IgG2B, Clone S395-68
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2B, IgG2b
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LabelPerCP
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Nav b 2 monoclonal IgG2B, clonality S395-68
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Alternative techniquemurine, antibodies
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Clone nameS395-68
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-68 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-68
- immunoglobulin lambda variable (I)-68 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-68 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 1-68
- immunoglobulin heavy variable 1-68 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameleucine carboxyl methyltransferase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 2 modulatory subunits
- 7BS protein methyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 68, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 68
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Locus
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Discovery year2011-08-08
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namecartilage acidic protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintegrator complex subunit 11
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Synonyms gene
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Synonyms gene name
- cleavage and polyadenylation specific factor 3-like
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Integrator complex
- MicroRNA protein coding host genes
- MBL fold containing DNA/RNA interacting subfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameD-aminoacyl-tRNA deacylase 1
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Synonyms gene
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Synonyms gene name
- chromosome 20 open reading frame 88
- D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae)
- D-tyrosyl-tRNA deacylase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-07-17
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data