Mouse Anti-Mouse Nav beta 2 Monoclonal IgG2B, Clone S395-68

• Catalog number
  SMC-485D
• Price
  Please ask
• Size
  100 µg

• Clone
  S395-68
• Immunogen
  Fusion protein amino acids 1-215 (full-length) of mouse NavBeta2
• Antibody s full description
  Mouse Anti-Mouse NaVbeta2 Monoclonal IgG2B Antibody, Clone: S395-68: Unconjugated
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  Sodium channel subunit beta-2 Antibody, Scn2b Antibody, UNQ326/PRO386 Antibody, Gm183 Antibody
• Raised in
  Mouse
• Antibody s target
  NaVbeta2
• Primary research fields
  Neuroscience, Ion Channels, Sodium Channels, Voltage-Gated Sodium Channels
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~40kDa. Does not cross-reat with NavBeta1, Navbeta3, or Navbeta4.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-485 was sufficient for detection of NavBeta2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  NavBeta2 or Scn2B, plays a crucial role in the assembly, expression and functional modulation of the heterotrimeric complex of the sodium channel. The subunit, beta-2 causes an increase in the plasma membrane surface area and in its folding into microvilli. It also interacts with TNR, playing a crucial role in clustering and regulation of activity of sodium channels at the nodes of Ranvier (1, 2). Mutations in this gene have been linked to Brugada syndrome and atrial fibrillation (3).
• Bibliography
  1. Genes and mapped phenotypes. (n.d.). Retrieved March 16, 2015, from more 2. Eubanks J., Srinivasan J., Dinulos M.B., Disteche C.M., Catterall W.A. (1997) Neuroreport. 8(12): 2775-2779. 3. Riuro H., et al. (2013) Hum Mutat. 34(7): 961-966.
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_001014761.1
• Gene number
  72821
• Protein number
  Q56A07
• Antibody s datasheet
  Contact our support service
• Representative figure link
  details | see here
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 2 Monoclonal Antibody, Clone S395-68 (SMC-485). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 2 Monoclonal Antibody (SMC-485) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Nav beta 2 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~40 kDa Nav-Beta-2 protein using Mouse Anti-Nav-Beta-2 Monoclonal Antibody, Clone S395-68 (SMC-485). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Nav-Beta-2 Monoclonal Antibody (SMC-485) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa. Mouse Anti-Nav beta 2 Antibody [S395-68] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-485) Mouse Anti-Nav-Beta-2 Antibody [S395-68] used in Western Blot (WB) on Rat Brain (SMC-485)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Nav   beta   Monoclonal   IgG2B   S395-68  
• Gene symbol
  IGLVI-68, IGHV1-68, LCMT1, RNU6-68P, CRTAC1, INTS11, DTD1
• Short name
  Mouse Anti-Mouse Nav beta 2 Monoclonal IgG2B, Clone S395-68
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2B, IgG2b
• Label
  Unconjugated
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse Nav b 2 monoclonal IgG2B, clonality S395-68
• Alternative technique
  murine, antibodies
• Clone name
  S395-68

Gene info

• Identity
  HGNC:5939
• Gene
  IGLVI-68
• Long gene name
  immunoglobulin lambda variable (I)-68 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (I)-68
  • immunoglobulin lambda variable (I)-68 pseudogene
• Synonyms
  • IGLV(I)-68
• GenBank acession
  • D86993
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28764
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000150853

Gene info

• Identity
  HGNC:5557
• Gene
  IGHV1-68
• Long gene name
  immunoglobulin heavy variable 1-68 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 1-68
  • immunoglobulin heavy variable 1-68 pseudogene
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28462
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151876

Gene info

• Identity
  HGNC:17557
• Gene
  LCMT1
• Long gene name
  leucine carboxyl methyltransferase 1
• Synonyms
  • CGI-68
  • PPMT1
• Synonyms name
  • protein phosphatase methyltransferase 1
  • [phosphatase 2A protein]-leucine-carboxy methyltransferase
• GenBank acession
  • AF037601
• Locus
  16p12.1
• Discovery year
  2003-01-29
• Entrez gene record
  51451
• Pubmed identfication
  • 10810093
• RefSeq identity
  • NM_016309
• Classification
  • Protein phosphatase 2 modulatory subunits
  • 7BS protein methyltransferases
• VEGA ID
  OTTHUMG00000177182

Gene info

• Identity
  HGNC:42558
• Gene
  RNU6-68P
• Long gene name
  RNA, U6 small nuclear 68, pseudogene
• Synonyms gene
  • RNU6-68
• Synonyms gene name
  • RNA, U6 small nuclear 68
• Locus
  13q14.13
• Discovery year
  2011-08-08
• Entrez gene record
  100873772
• RefSeq identity
  • NR_046938

Gene info

• Identity
  HGNC:14882
• Gene
  CRTAC1
• Long gene name
  cartilage acidic protein 1
• Synonyms
  • FLJ10320
  • CEP-68
  • ASPIC1
• GenBank acession
  • AJ276171
• Locus
  10q24.2
• Discovery year
  2001-05-11
• Entrez gene record
  55118
• Pubmed identfication
  • 11139377
• RefSeq identity
  • NM_018058
• VEGA ID
  OTTHUMG00000018871

Gene info

• Identity
  HGNC:26052
• Gene
  INTS11
• Long gene name
  integrator complex subunit 11
• Synonyms gene
  • CPSF3L
• Synonyms gene name
  • cleavage and polyadenylation specific factor 3-like
• Synonyms
  • FLJ20542
  • RC-68
  • CPSF73L
  • INT11
• GenBank acession
  • AL136813
• Locus
  1p36.33
• Discovery year
  2005-05-19
• Entrez gene record
  54973
• Pubmed identfication
  • 15684398
  • 16239144
• RefSeq identity
  • NM_017871
• Classification
  • Integrator complex
  • MicroRNA protein coding host genes
  • MBL fold containing DNA/RNA interacting subfamily
• VEGA ID
  OTTHUMG00000003330

Gene info

• Identity
  HGNC:16219
• Gene
  DTD1
• Long gene name
  D-aminoacyl-tRNA deacylase 1
• Synonyms gene
  • C20orf88
  • HARS2
• Synonyms gene name
  • chromosome 20 open reading frame 88
  • D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae)
  • D-tyrosyl-tRNA deacylase 1
• Synonyms
  • DUEB
  • MGC119131
  • MGC41905
  • bA379J5.3
  • bA555E18.1
  • pqn-68
• Synonyms name
  • DNA-unwinding element-binding protein B
• GenBank acession
  • AF332356
• Locus
  20p11.23
• Discovery year
  2001-07-17
• Entrez gene record
  92675
• RefSeq identity
  • NM_080820
• VEGA ID
  OTTHUMG00000031980

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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