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Gene info
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
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Gene names
NFKBIA, IKBA, MAD-3, NFKBI
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIA.
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Immuno application
ELISA, Western Blot
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Antibody advice
NFKBIA antibody can be used for detection of NFKBIA by ELISA at 1:1562500. NFKBIA antibody can be used for detection of NFKBIA by western blot at 0.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store NFKBIA antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human
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Omim nr
10092619
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Ncbi nr
NP_065390
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KDa
36 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
4792
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GENE symbol
NFKBIA
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Antigene name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
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Source
Homo sapiens
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Protein nr
P25963
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
NFKBIA
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Short name
anti-NFKBIA Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody tonuclear factor on kappa light polypeptide gene enhancer in B-cells inhibitor, a (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, IKBA and MAD-3 and NFKBI, NFKBIA and IDBG-4758 and ENSG00000100906 and 4792, NF-kappaB binding, nuclei, Nfkbia and IDBG-140708 and ENSMUSG00000021025 and 18035, BIKBA and IDBG-632547 and ENSBTAG00000016683 and 282291
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